Catalog Number - 64424-10P-2

Nova Similars Phenyl™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped phenyl bonded phase. The stationary phase consists of a four-carbon linker terminating in a phenyl functional group, providing unique chromatographic selectivity through a combination of hydrophobic and π-π interactions. Designed as a cost-effective alternative to Waters™ Nova-Pak® Phenyl columns, it offers comparable chromatographic behavior while delivering the reproducibility and performance required for demanding analytical methods.

64424-10P-2 is a 100 mm x 2.1 mm ID narrow-bore analytical column packed with 4 µm Nova Similars Phenyl particles. The 100 mm length provides an excellent balance between chromatographic resolution and analysis speed, while the 2.1 mm ID format minimizes solvent consumption and is particularly well suited for LC-MS applications. The 4 µm particle size provides improved efficiency and sharper peak shapes than comparable 5 µm columns while maintaining moderate operating pressure. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of reliable low-flow operation with low-volume plumbing and flow cells.

This column is particularly useful for aromatic compounds where phenyl selectivity can provide different retention and separation characteristics than traditional C18 phases. Common applications include pharmaceutical analysis, metabolite studies, environmental testing, aromatic hydrocarbons, and LC-MS methods requiring enhanced aromatic selectivity and sensitivity.

Key Benefits

• Type-B spherical silica stationary phase

• Fully endcapped phenyl bonded phase

• Enhanced aromatic compound selectivity

• Unique π-π interaction mechanism

• Narrow-bore format ideal for LC-MS applications

• Balanced speed and resolution with 100 mm length

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption

Typical Applications

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Aromatic compound separations

• Environmental testing

• Metabolite analysis

• Method development

• Quality control testing

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars Phenyl

• USP Classification: L11

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: Phenyl

• Endcapped: Fully

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Phenyl selectivity provides different retention than C18 phases

• Enhanced interactions with aromatic compounds

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

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